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0 Aldehydes are released from the breakdown of long-chain polyunsaturated fatty acids by free radical attacks. High levels of these aldehydes are found in a variety of diseases and abnormal metabolism states including coronary artery disease, diabetes, and Parkinson’s disease.
A reagent system is described herein for detecting the presence of aldehydes in such samples as blood, urine or other body fluids. The reagent includes Component A (pH from about 1.4 to 1.6) comprising bisulfite, pararosaniline HCl and hydrochloric acid; and Component B, a solution of hydroxide in water with a pH of about 10-11.
Aldehyde Dehydrogenase Assay Kit
The Aldehyde Dehydrogenase Assay Kit provides a quick and simple method to measure the activity of aldehyde dehydrogenase in different types of samples, such as tissue, cell lysate, plasma, and urine. It also allows for the screening of potential inhibitor compounds.
The aldehyde dehydrogenase enzyme family is an important group of NADH-dependent enzymes involved in the metabolism of a wide variety of aliphatic and aromatic aldehydes and ketones. The enzymes catalyze the oxidation of alcohols to produce acetaldehyde, acetate, and reactive oxygen species (ROS). Low enzyme activity produces excess acetaldehyde, which can result in oxidative stress and damage to cells and organs. In addition, the level of aldehyde dehydrogenase isoenzymes is a marker of liver disease in patients with alcoholic liver diseases [6,7].
Aldehyde dehydrogenase activity is detected using a coupled enzyme assay in which acetaldehyde is oxidized by ALDH to generate NADH. The resulting NADH reacts with a probe to generate a colorimetric product proportional to the amount of ALDH present in the sample.
Enzymes
These enzymes are essential for the biosynthesis of several metabolically important compounds including acetaldehyde, ethanoic acid, ethanol, and propionaldehyde. These enzymes are regulated by NAD+ and NADH in the cell and contribute to the maintenance of normal cellular metabolism and cellular differentiation.
However, these enzymes can also be found in malignant cells and can be a useful marker for the diagnosis and monitoring of Hepatitis B or Hepatitis C. In a study, the activity of alcohol dehydrogenase and its isoenzymes was measured in serum samples of patients with chronic hepatitis B or hepatitis C to determine the extent of liver cirrhosis. The results showed that total alcohol dehydrogenase and aldehyde dehydrogenase levels were higher in patients with alcoholic cirrhosis than those without cirrhosis, but there was no statistically significant difference between the groups.
The aldehyde dehydrogenase family also contains a subfamily of aldehyde dehydrogenases called the nitric oxide synthases, which are important for the regulation of cell growth and development during placental and uterine development. Moreover, the activity of nitric oxide synthase is related to the development and function of human embryonic stem cells. Inhibitors of nitric oxide synthase have been shown to suppress the development of several tumor types and improve outcomes in cancer treatment.
Aldehyde Dehydrogenase Activity Colorimetric Assay Kit
Aldehyde test kit Dehydrogenases (ALDHs) catalyze the oxidation of endogenous and exogenous aldehydes to their corresponding carboxylic acids. These enzymes are involved in a wide variety of cellular processes and have been linked to numerous diseases.
The aldehyde test kit acetaldehyde dehydrogenase activity colorimetric assay aldehyde test kit provides a simple and direct procedure for measuring the activity of ALDH in various samples. The test is based on a coupled enzyme assay in which acetaldehyde is oxidized by ALDH generating NADH, which reacts with a probe generating a colorimetric (450 nm) product proportional to the activity present.
Detection of ALDH activity in tissue homogenates, cell culture supernatants, and lysates is simple and quick with this kit. The assay is compatible with high-throughput handling systems and is suitable for both quantitative and qualitative measurements of ALDH activity.
This ALDH activity assay has a detection range from 5 pg/ml to 1 nmol/ml. Samples should be stored at -20 degC or below to maintain bioactivity and to avoid contamination and loss of reaction time.
Inactivation of aldehyde
The inactivation of aldehyde dehydrogenase by ethanol is often associated with diseases such as cancer, cataracts, and heart disease. This test can be used to monitor the activities of aldehyde dehydrogenases in a variety of samples to ensure the safety of patients with these conditions.
Use of this aldehyde test kit should be done with caution and in the presence of certain chemicals such as methanol and acetone. This is because these may impact the ELISA results and can potentially result in false positive results.
Preparation of the test is straightforward and requires only a few steps: reconstituted Standards or Detection Reagents A and B should be pipetted into good strips; well strips should then be secured in a microtiter plate. During incubation, wells should not be allowed to remain uncovered for long periods.
Reconstituted reagents should be used only once, as they are prone to degradation due to heat exposure. If these reagents are stored for longer than 15 days, they should be recleared in water to ensure proper shelf life.
This Aldehyde activity assay has high sensitivity and specificity for the identification of ALDH6 family members. No significant cross-reactivity or interference with analogs was observed.
Aldehyde Dehydrogenase Activity Detection Kit
The aldehyde dehydrogenase (ALDH) family is a superfamily of intracellular enzymes that catalyze the conversion of numerous diverse physiologically and pathophysiologically relevant aldehydes to their corresponding non-toxic carboxylic acids. Although primarily involved in acetaldehyde detoxification and cytoprotective biological roles, ALDH is also known to regulate cell proliferation, differentiation, and survival.
Several alcoholic diseases are associated with altered levels of ALDH in the liver. Imbalances of this enzyme can lead to hepatotoxicity, and in turn, result in several different types of malignancies. Increased ALDH activity is a cancer stem cell marker and is correlated with poor prognosis, making it important to detect early.
A simple, fast, and robust method for measuring the enzymatic activity of aldehyde dehydrogenase is available in the ALDH Activity Colorimetric Assay Kit. This aldehyde test kit is designed for high throughput analysis and provides a sensitive assay that can detect 0.5 mU of enzymatic activity in a variety of samples.
The kit uses for formazan Reagent
This kit uses a formazan reagent that has been reduced to a colored product by the enzyme activity. The resulting color is proportional to the ALDH activity. It is a rapid, easy-to-use assay that can be used in any lab.
In this colorimetric assay, acetaldehyde is oxidized by aldehyde dehydrogenase to produce NADH which reduces the formazan reagent to a colored product. The amount of reagent in the test sample is then measured against that in a control sample.
For optimal excitation and emission of the reagent, a flow cytometer with a 488 nm laser and appropriate filters is required. The reagent can be detected in proximal measurement channels, but the delta MFI between the test and control sample may vary.
This fluorometric assay is 10 times more sensitive than the colorimetric assay and can detect 0.05 mU of enzymatic activity (based on our unit definition). It is designed for high throughput analysis and can be used in any lab.
Aldehyde Dehydrogenase Activity Quantitative Assay Kit
Aldehyde Dehydrogenase (ALDH) is an essential enzyme that detoxifies and oxidizes endogenous and exogenous reactive aldehydes such as 4-hydroxy-2-nominal, which are produced by the cellular metabolism of lipid peroxidation. Its subfamilies have been shown to play an important role in the physiology and pathogenesis of a variety of malignant cells, including cancers of the breast, lung, prostate, and ovary, as well as in the development and survival of patients with alcoholism and cardiac ischemia [17-20].
The ALDH1A2 enzyme is also known to be involved in the synthesis of retinoic acid, which serves as a potent signaling molecule for stem cell differentiation. Retinoic acid is a key component of the retinoic acid receptor and retinoic X receptor heterodimers, which regulate the transcription of target genes essential for stem cell development. It also binds and activates nuclear retinoic acid receptor/retinoic X receptor complexes to promote cytokines, which in turn control cell growth and differentiation.
Quantitative measurement
In vitro quantitative measurement of ALDH activity in human tissue homogenates, cell lysates, and other biological fluids. Suitable for use with standard cell sorters and analyzers.
Compared to most other assays, this is an extremely sensitive and robust method of monitoring He activity in a wide range of sample types including tissues, cell lysates, and plasma. It can also be used for screening potential He inhibitor compounds.
This kit is based on the aldehyde test kit dehydrogenase-catalyzed oxidation of acetaldehyde, which can convert a nearly colorless probe to an intensely colored product. The resulting NADH can be monitored using a spectrophotometer at 340 nm. The extinction coefficient is approximately 6.22 mM-1 cm-1.
Purpald® Kit (R)
CHEMetrics offers formaldehyde test kits that use the Purpald® method, which provides a purple color that is directly related to the concentration of formaldehyde present in the sample. The color is measured as ppm (mg/L) of CH2O and results are reported in a report that includes information on recommended actions to take based on the findings.
Amplite® Kit, Blue
The Amplite® Colorimetric Aldehyde Quantitation Assay is designed to provide a rapid, simple and direct method for detecting aldehydes in a wide range of samples. The assay utilizes a reagent solution that reacts with the presence of aldehyde to produce a colorimetric product at a wavelength of 620 nm. This colorimetric method is able to detect up to 0.3 nanomoles of aldehyde per 100 mL assay volume.
Neutralex® Formalin and Aldehyde Waste Test Kit
The Neutralex® Formalin and Aldehyde waste test kit is designed for a fast and reliable semi-quantitative determination of residual formalin and pH in neutralex treated waste. It tests for a specific concentration of formalin and pH using a simple procedure that is completed in 20 seconds.
Environmental Diagnostics Laboratory® HI3838
The HI3838 is an automated, titration-based formaldehyde test kit that allows for measurement of the formaldehyde concentration in two ranges: 0.0 to 1.0% and 0 to 10%. The kit consists of a beaker, indicator and reagent bottles, and a calibrated syringe for performing the analysis.
This test can be used to analyze air, wastewater, and drinking water for the presence of formaldehyde. It can also be used to monitor a variety of other pollutants, such as volatile organic compounds and nitrogen dioxide.
EDL’s Analytical Services
When you purchase an aldehyde test kit, it will include a pre-addressed envelope for sending the sample to Environmental Diagnostics Laboratory (EDL). Once your sample has been received by EDL, your test will be performed and a report will be sent to you within 3-5 business days.
The EDL analytical services staff will review your sample and send you a report with details on the level of formaldehyde that was detected. You will also receive a summary of recommendations on how to proceed with remediation. You can reach the lab staff by phone or email with any questions or concerns you have about your formaldehyde testing results.
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